| Gene | Neurod2 |
| Chromosome | 11 |
| Deletion Start | 98,328,333 |
| Deletion End | 98,327,160 |
| Deletion Size | 1,174 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,327,905 | 98,327,922 | AACGCGGCTCTGGACAAC |
| TUP | 98,327,879 | 98,327,901 | CGCAAGGTGGTGCCCTGCTACTC |
| TUR | 98,327,842 | 98,327,860 | GCGCAGGGTCTCGATCTTG |
| TDF | 98,327,725 | 98,327,744 | CACAGCCCACCACGAATCTG |
| TDP | 98,327,699 | 98,327,720 | CCGGCTGCCTGCAGCTAAACTC |
| TDR | 98,327,608 | 98,327,626 | CGGGTATGGGTACGGATGC |
PCR Primers
| Primer | Sequence |
| SU | CTGGTCAAGCTCTCTGGGAC |
| SD | CTATCCCGAGACTCAGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15635A-F8 | VGB6 | C57BL/6NTac | |
| 15635A-G5 | VGB6 | C57BL/6NTac | |
| 15635B-D6 | VGB6 | C57BL/6NTac | |
| 15635B-G1 | VGB6 | C57BL/6NTac | |
