| Gene | Krt26 |
| Chromosome | 11 |
| Deletion Start | 99,337,901 |
| Deletion End | 99,329,596 |
| Deletion Size | 8,306 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,337,547 | 99,337,566 | GGGCTGGTACGAGAAGTGTG |
| TUP | 99,337,521 | 99,337,543 | CCGGCAGAGGCCGTAGACTTGAC |
| TUR | 99,337,458 | 99,337,482 | CTCTGCATTCCTTACCTGTCGTTTC |
| TDF | 99,329,764 | 99,329,787 | AGACTCAGCAGAAGAAACATTTGC |
| TDP | 99,329,742 | 99,329,763 | CAGAACAGTGGCTGAAGAGCTA |
| TDR | 99,329,712 | 99,329,735 | TCTCAGTGAAAGGAGGTTACCAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTTGGTAACCCAGGTAATGG |
| SD | AAGCCCACATTGATGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15637A-A11 | VGB6 | C57BL/6NTac | |
| 15637A-C1 | VGB6 | C57BL/6NTac | |
| 15637A-D2 | VGB6 | C57BL/6NTac | |
| 15637A-E3 | VGB6 | C57BL/6NTac | |
| 15637A-C6 | VGB6 | C57BL/6NTac | |
