| Gene | Stat5a |
| Chromosome | 11 |
| Deletion Start | 100,884,040 |
| Deletion End | 100,861,341 |
| Deletion Size | 22,700 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,861,506 | 100,861,529 | GGTCTCCTCTTGTGGTTTGGTATG |
| TUP | 100,861,532 | 100,861,557 | TCTGGTTCTATCTGTCCTTGTGCGGA |
| TUR | 100,861,582 | 100,861,604 | TGATAGCTCCAGTTTCCCTCTTC |
| TDF | 100,883,396 | 100,883,415 | CACTACCACACCCAGCCAAG |
| TDP | 100,883,419 | 100,883,445 | TAGAGTCTGATTCTAGCTGCAGGTGGC |
| TDR | 100,883,483 | 100,883,505 | GACCACTAATCCTCCAAACCATC |
PCR Primers
| Primer | Sequence |
| SU | GTCTAGTTAGGGACTGTG |
| SD | AGTTGCCTAAACCTGCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15641A-E5 | VGB6 | C57BL/6NTac | |
| 15641A-D11 | VGB6 | C57BL/6NTac | |
| 15641A-A7 | VGB6 | C57BL/6NTac | |
| 15641A-G7 | VGB6 | C57BL/6NTac | |
| 15641A-F6 | VGB6 | C57BL/6NTac | |
