| Gene | Adam11 |
| Chromosome | 11 |
| Deletion Start | 102,778,261 |
| Deletion End | 102,761,624 |
| Deletion Size | 16,638 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,762,375 | 102,762,393 | TCACAGCCCACAACAGTCC |
| TUP | 102,762,395 | 102,762,420 | CCAAGTAGGCAGGGTTAGGTGCCACC |
| TUR | 102,762,435 | 102,762,457 | ACAGAAAGGGAAGCTGCGGTAAG |
| TDF | 102,777,297 | 102,777,317 | TGAAGACTGCTCAACGCATCC |
| TDP | 102,777,318 | 102,777,339 | CAGCACGGCCTAGGGCTTGTCT |
| TDR | 102,777,362 | 102,777,382 | GGAGTTCCTGGGCTGAATGTG |
PCR Primers
| Primer | Sequence |
| SU | AGACCTCAGGGACCCTCTTC |
| SD | CCTTCCCGTGAGGTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15643A-B2 | VGB6 | C57BL/6NTac | |
| 15643B-A9 | VGB6 | C57BL/6NTac | |
| 15643B-B6 | VGB6 | C57BL/6NTac | |
| 15643B-C5 | VGB6 | C57BL/6NTac | |
| 15643C-D3 | VGB6 | C57BL/6NTac | |
