| Gene | Gfap |
| Chromosome | 11 |
| Deletion Start | 102,897,114 |
| Deletion End | 102,888,600 |
| Deletion Size | 8,515 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,896,188 | 102,896,210 | GCTGTAGAGGTCAGGAAACAATC |
| TUP | 102,896,153 | 102,896,180 | AAAGATCGGAACCAAGGGTCACACTCCA |
| TUR | 102,896,123 | 102,896,142 | ATGCCCACCACCCAGCAAAG |
| TDF | 102,889,499 | 102,889,520 | AAACCAGCCTGGACACCAAATC |
| TDP | 102,889,473 | 102,889,496 | TGTCAGAAGGCCACCTCAAGAGGA |
| TDR | 102,889,443 | 102,889,464 | CCCGCATCTCCACAGTCTTTAC |
PCR Primers
| Primer | Sequence |
| SU | GGTTCCTTGATCAATGGC |
| SD | CTTCCAGCTCTGGCAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15646A-A9 | VGB6 | C57BL/6NTac | |
| 15646A-B4 | VGB6 | C57BL/6NTac | |
| 15646A-B11 | VGB6 | C57BL/6NTac | |
| 15646A-E2 | VGB6 | C57BL/6NTac | |
| 15646A-E4 | VGB6 | C57BL/6NTac | |
