15649   Kpna2     Status: ES cell colonies screened / QC positives

GeneKpna2
Chromosome11
Deletion Start106,997,309
Deletion End106,988,887
Deletion Size8,423
Genome Build38
MGI IDMGI:103561
NCBI Gene ID16647
BAC ID58G20
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,997,289106,997,309TCCACGAACGAGAATGCTAAC
TUP106,997,264106,997,288TTACCAGCTGCCCGACTTAACAGGT
TUR106,997,208106,997,226GCACGCAGGAAAGCAACAG
TDF106,989,700106,989,721GGTGGAGCAAGCCTTTAATTCC
TDP106,989,671106,989,696ACTCAGGAGGAAGATGCAGGCAGATC
TDR106,989,638106,989,660GCCATGTAGACTAGGCTGATCTC

PCR Primers

PrimerSequence
SUAGTATAGAAATGGAGTTGAG
SDTACCGGTAACACATCCAGGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR102 bp++-
TDFTDR84 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15649A-C6VGB6C57BL/6NTac
15649A-H5VGB6C57BL/6NTac
15649A-F8VGB6C57BL/6NTac
15649A-E3VGB6C57BL/6NTac
15649A-E12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image