| Gene | Prkar1a |
| Chromosome | 11 |
| Deletion Start | 109,667,643 |
| Deletion End | 109,653,788 |
| Deletion Size | 13,856 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,653,794 | 109,653,814 | GGCAGTATGGCAACCAGTGAG |
| TUP | 109,653,824 | 109,653,849 | AGTCTCCGGGAATGCGAGCTCTATGT |
| TUR | 109,653,851 | 109,653,872 | GGGCCTGGATATTGTGCTTCTG |
| TDF | 109,667,171 | 109,667,189 | CCTCGACTGCCTGGACTTC |
| TDP | 109,667,199 | 109,667,226 | TGAAGCTTGTTCTAACTGGTCACCTTCC |
| TDR | 109,667,241 | 109,667,262 | TCATGCCTACAAACCAACACTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTCTGACCTGCTTCCCAC |
| SD | TACAGCAAACATGGCCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15651B-C11 | VGB6 | C57BL/6NTac | |
| 15651B-G1 | VGB6 | C57BL/6NTac | |
| 15651B-H9 | VGB6 | C57BL/6NTac | |
| 15651C-B1 | VGB6 | C57BL/6NTac | |
| 15651C-B3 | VGB6 | C57BL/6NTac | |
| 15651C-B6 | VGB6 | C57BL/6NTac | |
