| Gene | Nat9 |
| Chromosome | 11 |
| Deletion Start | 115,186,822 |
| Deletion End | 115,182,971 |
| Deletion Size | 3,852 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,186,784 | 115,186,803 | TGCTAGTGGGCAAGAAGGTG |
| TUP | 115,186,759 | 115,186,782 | TCCTGGTACCCTACACCTCCGAGC |
| TUR | 115,186,711 | 115,186,730 | CCTCCACGCTACAACCAGTC |
| TDF | 115,183,889 | 115,183,908 | TGTGCGTCCTTGGTCCTTAG |
| TDP | 115,183,865 | 115,183,888 | CTTCTGCCAAGAGCTCCCTGGTGA |
| TDR | 115,183,836 | 115,183,854 | GGCTCCCAGCAATCTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCCTGAGAACTTTGAGTGGG |
| SD | CCTCTCTGACAGGGATGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15652A-B8 | VGB6 | C57BL/6NTac | |
| 15652A-C7 | VGB6 | C57BL/6NTac | |
