| Gene | Tmem104 |
| Chromosome | 11 |
| Deletion Start | 115,205,578 |
| Deletion End | 115,188,281 |
| Deletion Size | 17,298 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,188,644 | 115,188,664 | GCTCAGTGTAAGTGCTGGAAG |
| TUP | 115,188,665 | 115,188,686 | TGAACTCGGGTCCTCTGCAGGA |
| TUR | 115,188,714 | 115,188,734 | GGAGAACTGGCCTGGAGAAAC |
| TDF | 115,205,319 | 115,205,339 | TCCAAAGGCACGGTCTGTATC |
| TDP | 115,205,340 | 115,205,362 | CCCAGACCTGTGAGCCATTGTGC |
| TDR | 115,205,393 | 115,205,415 | AGCTCGGACTTCCCACAAACTAC |
PCR Primers
| Primer | Sequence |
| SU | CGGACGTAAGCACTTTAGGG |
| SD | TCACTGGCGTGGAGTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15653A-A12 | VGB6 | C57BL/6NTac | |
| 15653B-B5 | VGB6 | C57BL/6NTac | |
