15654   Grin2c     Status: ES cell colonies screened / QC positives

GeneGrin2c
Chromosome11
Deletion Start115,261,129
Deletion End115,249,569
Deletion Size11,561
Genome Build38
MGI IDMGI:95822
NCBI Gene ID14813
BAC ID274G12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF115,260,710115,260,734GGTGCCTGTCTATAATTCTCATCTC
TUP115,260,684115,260,708TCCTCCATGAGCCCACAACTAGCTA
TUR115,260,649115,260,671AAGCTGCTCCGTAATTCAAACTC
TDF115,250,010115,250,027CGTGGCAGAAGCCTTCAC
TDP115,249,988115,250,009TCGATCCAACCCTCTGCCTGCC
TDR115,249,903115,249,921GCCGCAACGACTGTGTTTG

PCR Primers

PrimerSequence
SUTCTTTGTTAGGGTAGAACCCAG
SDGAGGCAGCTAGGACCAAATG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR86 bp++-
TDFTDR125 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15654A-A5VGB6C57BL/6NTac
15654A-D6VGB6C57BL/6NTac
15654A-E2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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