15656   Sap30bp     Status: ES cell colonies screened / QC positives

GeneSap30bp
Chromosome11
Deletion Start115,964,450
Deletion End115,933,679
Deletion Size30,772
Genome Build38
MGI IDMGI:1927479
NCBI Gene ID57230
BAC ID87K11
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF115,934,486115,934,507GCTGGTTCAAAGAGGAGAAGAC
TUP115,934,511115,934,538AACTGCCCTAATAGGCCAACAGCATGTA
TUR115,934,552115,934,571GGTTCCCAGCAGTTCCAAAG
TDF115,964,152115,964,170CTGCCTCTGGGTCCTGTTG
TDP115,964,173115,964,199TGGTGCTGATCTTGACCCATCTCTTGT
TDR115,964,216115,964,237CCCACTTGCTCTTCCTCTTCTG

PCR Primers

PrimerSequence
SUGCTCTCCAAGATGTCAGAC
SDCTATGGCCTGTCAAGCACTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR86 bp++-
TDFTDR86 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15656A-G6VGB6C57BL/6NTac
15656B-B4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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