| Gene | Srp68 |
| Chromosome | 11 |
| Deletion Start | 116,274,103 |
| Deletion End | 116,245,631 |
| Deletion Size | 28,473 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,273,925 | 116,273,945 | GGACAGCCTGAGTTTGGAGAG |
| TUP | 116,273,900 | 116,273,923 | ATCCTCGAGCGGTGGCCTAACTCG |
| TUR | 116,273,879 | 116,273,899 | CCGAGCGACACGTAGTCATTG |
| TDF | 116,245,755 | 116,245,777 | GAGGACAAATTGGAGCAGAAGAC |
| TDP | 116,245,729 | 116,245,753 | AAGAGTGGCCTCACAGGCTACATCA |
| TDR | 116,245,686 | 116,245,706 | CCCGAGGAAGAGCCTGGTTAG |
PCR Primers
| Primer | Sequence |
| SU | CGGGTCTCAAGCTCCTATTG |
| SD | AGAGCGCTCTGCTTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15658A-A8 | VGB6 | C57BL/6NTac | |
| 15658B-B6 | VGB6 | C57BL/6NTac | |
| 15658B-F11 | VGB6 | C57BL/6NTac | |
| 15658C-C7 | VGB6 | C57BL/6NTac | |
| 15658C-C9 | VGB6 | C57BL/6NTac | |
| 15658C-D8 | VGB6 | C57BL/6NTac | |
| 15658C-F9 | VGB6 | C57BL/6NTac | |
| 15658A-B8 | VGB6 | C57BL/6NTac | |
