| Gene | Foxa1 |
| Chromosome | 12 |
| Deletion Start | 57,545,732 |
| Deletion End | 57,542,021 |
| Deletion Size | 3,712 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,545,005 | 57,545,024 | GCATTGCGAGGAGAATCAGG |
| TUP | 57,544,979 | 57,545,003 | ACAGGCAGGGCCACTACCTATTGAA |
| TUR | 57,544,897 | 57,544,917 | GCACGAGGCTTTCTCGGTAAG |
| TDF | 57,542,197 | 57,542,215 | GCAACAGCACAAGCTGGAC |
| TDP | 57,542,173 | 57,542,195 | TCAAGGCATACGAGCAGGCGCTG |
| TDR | 57,542,143 | 57,542,162 | GGGCAAGGTAGCGCCATAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCTCGGGCTTTGTAGGTG |
| SD | GCCACAGACCTGTAAACTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15664A-A7 | VGB6 | C57BL/6NTac | |
| 15664A-A9 | VGB6 | C57BL/6NTac | |
| 15664A-C6 | VGB6 | C57BL/6NTac | |
| 15664A-D4 | VGB6 | C57BL/6NTac | |
| 15664A-F3 | VGB6 | C57BL/6NTac | |
