| Gene | Fkbp3 |
| Chromosome | 12 |
| Deletion Start | 65,073,919 |
| Deletion End | 65,062,675 |
| Deletion Size | 11,245 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,073,611 | 65,073,629 | GGGTTCGGCCCTTATTTCC |
| TUP | 65,073,578 | 65,073,601 | CCCTGGAGCTGCGGCCGTAGATTT |
| TUR | 65,073,518 | 65,073,539 | CCAGCATTTCAGGGTCCTTCTC |
| TDF | 65,063,551 | 65,063,572 | TGGCATGTTGTGGGTATGTATG |
| TDP | 65,063,524 | 65,063,550 | TGCTCACAGAAGCAATCCAACAAACCA |
| TDR | 65,063,497 | 65,063,521 | GGAAGACTATCTGGAAAGGAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | CCAAAGAACGCCTCAGAAAG |
| SD | CCTTGACAAAGCTAGCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15665A-D12 | VGB6 | C57BL/6NTac | |
| 15665A-C1 | VGB6 | C57BL/6NTac | |
| 15665A-D7 | VGB6 | C57BL/6NTac | |
| 15665A-C11 | VGB6 | C57BL/6NTac | |
| 15665A-B11 | VGB6 | C57BL/6NTac | |
| 15665A-H4 | VGB6 | C57BL/6NTac | |
