15666 Actr10 Status: ES cell colonies screened / QC positives
| Gene | Actr10 |
| Chromosome | 12 |
| Deletion Start | 70,962,048 |
| Deletion End | 70,937,958 |
| Deletion Size | 24,091 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,938,628 | 70,938,646 | AAGCACGGGAGTAGTGGTC |
| TUP | 70,938,649 | 70,938,677 | CGAAGTGGCAGTCAAAGGTAGAGATAGCA |
| TUR | 70,938,713 | 70,938,734 | ACCGTCCAGCATCCTCTCATAC |
| TDF | 70,961,720 | 70,961,740 | TCATGGCTCACCCTGTTTCTC |
| TDP | 70,961,750 | 70,961,778 | CTCATTGAGTTGGAGTTGAGAGCTTGGTC |
| TDR | 70,961,799 | 70,961,820 | GTGTAAGCTACGCAAAGAGGAG |
PCR Primers
| Primer | Sequence |
| SU | AATTAAGGAGCATTTCCGCC |
| SD | CCTGAAATGACTGCAAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
