| Gene | Psen1 |
| Chromosome | 12 |
| Deletion Start | 83,734,168 |
| Deletion End | 83,699,568 |
| Deletion Size | 34,601 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,700,092 | 83,700,111 | CCCTGTCAGCTAAGCACTAC |
| TUP | 83,700,119 | 83,700,139 | AGGCAGACTCCACAGCAGGCC |
| TUR | 83,700,171 | 83,700,193 | CCTCCATCTTGGTTGTACCTCAG |
| TDF | 83,733,636 | 83,733,656 | TGCATCTCCTCCTGTCTTCTC |
| TDP | 83,733,657 | 83,733,677 | TTTCCCGTCTCCCTCGAGCCA |
| TDR | 83,733,679 | 83,733,699 | CAGGCTGGACAACAGCGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCAACAGGTACAAGTGGA |
| SD | CACAGTGCGGGTAAATCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15671A-B11 | VGB6 | C57BL/6NTac | |
| 15671A-B12 | VGB6 | C57BL/6NTac | |
| 15671A-D10 | VGB6 | C57BL/6NTac | |
| 15671A-F5 | VGB6 | C57BL/6NTac | |
| 15671A-E2 | VGB6 | C57BL/6NTac | |
