| Gene | Vipar |
| Chromosome | 12 |
| Deletion Start | 87,263,994 |
| Deletion End | 87,239,721 |
| Deletion Size | 24,274 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,263,890 | 87,263,912 | TCTCACAGGTAGGTTTCCACAAG |
| TUP | 87,263,853 | 87,263,881 | AAGGGTCATACCATACTCTTGGATGCGTC |
| TUR | 87,263,819 | 87,263,841 | ACGAGCCTACTGGAGATGAGAAG |
| TDF | 87,239,841 | 87,239,862 | GCCTTCCTTGTCTGCTTTCTTC |
| TDP | 87,239,810 | 87,239,839 | CAGCAAATTCGATGGAAGAACTAAGGGTCC |
| TDR | 87,239,786 | 87,239,805 | GCGAGGAGGCTTCAGGGTAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTACAACATTTGCCTCTCG |
| SD | GAAATGCCTATGCCCAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15673A-B2 | VGB6 | C57BL/6NTac | |
| 15673A-B4 | VGB6 | C57BL/6NTac | |
| 15673A-C5 | VGB6 | C57BL/6NTac | |
| 15673A-E3 | VGB6 | C57BL/6NTac | |
| 15673A-F1 | VGB6 | C57BL/6NTac | |
