| Gene | Gpr132 |
| Chromosome | 12 |
| Deletion Start | 112,855,839 |
| Deletion End | 112,851,957 |
| Deletion Size | 3,883 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,855,701 | 112,855,719 | CCTGAGCAAGCAGCGAGAG |
| TUP | 112,855,674 | 112,855,698 | AGAGCCGTGCCAGGGAATCTGTTCA |
| TUR | 112,855,655 | 112,855,673 | GCCTTTCCCAGAACCACAC |
| TDF | 112,852,488 | 112,852,508 | GCGCAGAAGAACAAGGTGAAG |
| TDP | 112,852,466 | 112,852,485 | CTCCGCCATCGCGGTCGTCA |
| TDR | 112,852,444 | 112,852,464 | GCAAAGCAGACCAGGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | GGCTGGCTTATTCTCATTG |
| SD | TAGGAGCTGGATCAATTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15680A-A5 | VGB6 | C57BL/6NTac | |
| 15680A-H1 | VGB6 | C57BL/6NTac | |
| 15680A-B4 | VGB6 | C57BL/6NTac | |
| 15680A-C6 | VGB6 | C57BL/6NTac | |
| 15680A-A4 | VGB6 | C57BL/6NTac | |
