| Gene | Gtpbp4 |
| Chromosome | 13 |
| Deletion Start | 8,995,993 |
| Deletion End | 8,973,080 |
| Deletion Size | 22,914 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,995,446 | 8,995,467 | AGCCAGGCCCACATTATGTAAC |
| TUP | 8,995,415 | 8,995,443 | AGCAAGATGCTTTCCTCCCAAATGTCGAG |
| TUR | 8,995,322 | 8,995,345 | GACACTGGTTCCTCTAACTGATTC |
| TDF | 8,974,025 | 8,974,045 | TTCACTCCACCTTCCACTTTG |
| TDP | 8,973,998 | 8,974,023 | AGCGTCCTTTGGAGAGGAACTTGGAC |
| TDR | 8,973,954 | 8,973,976 | GTTTGCCAGACAGAACACCTATC |
PCR Primers
| Primer | Sequence |
| SU | CTTGGGTGAGGGCAGTGT |
| SD | AGCCTTCTGCCTAGACCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 146 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15683A-B2 | VGB6 | C57BL/6NTac | |
| 15683A-G11 | VGB6 | C57BL/6NTac | |
| 15683A-G6 | VGB6 | C57BL/6NTac | |
