| Gene | Mrpl32 |
| Chromosome | 13 |
| Deletion Start | 14,613,015 |
| Deletion End | 14,610,468 |
| Deletion Size | 2,548 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,612,139 | 14,612,163 | GGATCAGGATAGTGTGGTACAGATG |
| TUP | 14,612,115 | 14,612,137 | CCATTGTAGTCATCTCAGTGATG |
| TUR | 14,612,089 | 14,612,111 | ATCCCGAGACTCCCTACATTCAC |
| TDF | 14,611,397 | 14,611,416 | TGTGAACGGGTGTAGGTGTG |
| TDP | 14,611,360 | 14,611,389 | ACACACTCTCTTCCACAAGTCTAACACTGA |
| TDR | 14,611,289 | 14,611,311 | TGAGCCAAACAAGATGCTAAGAC |
PCR Primers
| Primer | Sequence |
| SU | GTCAGCGAGAAGCTGTAAC |
| SD | GTGTACCACATACCTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 128 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15684A-A2 | VGB6 | C57BL/6NTac | |
| 15684A-B3 | VGB6 | C57BL/6NTac | |
| 15684A-E8 | VGB6 | C57BL/6NTac | |
| 15684A-F11 | VGB6 | C57BL/6NTac | |
