| Gene | Tifab |
| Chromosome | 13 |
| Deletion Start | 56,176,625 |
| Deletion End | 56,176,143 |
| Deletion Size | 483 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,176,453 | 56,176,475 | CAGTTGTCTCGCTACCATTTGTC |
| TUP | 56,176,427 | 56,176,450 | TGGAACCCTACCTGGAGAAAGGCA |
| TUR | 56,176,394 | 56,176,413 | TCAGCACCTTGAGGCAGAAG |
| TDF | 56,176,253 | 56,176,272 | AGCCTCCCTTGAGACCTTTG |
| TDP | 56,176,225 | 56,176,250 | TGCTACTTCCATCTCAGCCCTTCACC |
| TDR | 56,176,177 | 56,176,197 | TTCCCGGTTCATTCATCTGTC |
PCR Primers
| Primer | Sequence |
| SU | CTGCCAGATTCAAGAAAGCC |
| SD | CGGAGGGCATTTCTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15698A-C4 | VGB6 | C57BL/6NTac | |
| 15698A-D4 | VGB6 | C57BL/6NTac | |
| 15698A-E8 | VGB6 | C57BL/6NTac | |
