15702 Cts6 Status: ES cell colonies screened / QC one positive
| Gene | Cts6 |
| Chromosome | 13 |
| Deletion Start | 61,202,262 |
| Deletion End | 61,195,348 |
| Deletion Size | 6,915 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,202,216 | 61,202,233 | TTTGGGAGTGGGCTCAGG |
| TUP | 61,202,190 | 61,202,215 | TGCCCTAGCACTTGATCCCAATTTGA |
| TUR | 61,202,162 | 61,202,186 | TGTTTCTTCCAATCATGCCATTCAG |
| TDF | 61,196,267 | 61,196,291 | CTAGAGAGATAGGTCAGTGGTTGAG |
| TDP | 61,196,239 | 61,196,262 | TTGGCTGCCCTTCTAGGAGACCCA |
| TDR | 61,196,216 | 61,196,235 | GACTGCAACGGGCAATTTTG |
PCR Primers
| Primer | Sequence |
| SU | TTTACTTTCTGGCCTCCCTG |
| SD | CACCAGCAACAAATTCACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
