| Gene | Otp |
| Chromosome | 13 |
| Deletion Start | 94,884,010 |
| Deletion End | 94,875,773 |
| Deletion Size | 8,238 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,876,599 | 94,876,618 | GAGGTGGAAGGCTTGGAGAC |
| TUP | 94,876,627 | 94,876,654 | CTTCCGAAAGTCAGAGGGAATTGGCCAG |
| TUR | 94,876,656 | 94,876,676 | GAGCAAAGGCGCTAAATGAAC |
| TDF | 94,883,048 | 94,883,068 | CGTGGAGTCTTGTCTCCCTTC |
| TDP | 94,883,078 | 94,883,097 | CGCCCGCTGCAGGTCTGGTT |
| TDR | 94,883,113 | 94,883,133 | TTCTTGCGCTTCTTCCACTTG |
PCR Primers
| Primer | Sequence |
| SU | TTTAGCGGCTGCTGGAGG |
| SD | TGTGCTGTACCTTGAACACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15708A-F10 | VGB6 | C57BL/6NTac | |
| 15708A-D9 | VGB6 | C57BL/6NTac | |
| 15708A-A9 | VGB6 | C57BL/6NTac | |
| 15708A-E5 | VGB6 | C57BL/6NTac | |
