| Gene | Aggf1 |
| Chromosome | 13 |
| Deletion Start | 95,375,055 |
| Deletion End | 95,351,496 |
| Deletion Size | 23,560 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,374,696 | 95,374,713 | GGGCAGGCGAGAATGAAC |
| TUP | 95,374,668 | 95,374,694 | TCAGCATGGGCTACATGGTCTTGATCG |
| TUR | 95,374,644 | 95,374,661 | TGCGCCCATTGCCAGTAG |
| TDF | 95,352,244 | 95,352,264 | GCTGTCCTGGAACTCGACTTG |
| TDP | 95,352,214 | 95,352,242 | ACACTAGGATGGCCTTGAACTCTCAGAGA |
| TDR | 95,352,185 | 95,352,205 | CCAGCACTAGGGACCCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | AACTAGAGGCTTCCGCTGG |
| SD | GCTCCACTACCCATGGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15709A-C8 | VGB6 | C57BL/6NTac | |
| 15709A-H4 | VGB6 | C57BL/6NTac | |
| 15709A-H10 | VGB6 | C57BL/6NTac | |
| 15709A-D3 | VGB6 | C57BL/6NTac | |
| 15709A-G3 | VGB6 | C57BL/6NTac | |
| 15709A-E9 | VGB6 | C57BL/6NTac | |
