15713   Rab3c     Status: ES cell colonies screened / QC positives

GeneRab3c
Chromosome13
Deletion Start110,280,015
Deletion End110,260,484
Deletion Size19,532
Genome Build38
MGI IDMGI:1914545
NCBI Gene ID67295
BAC ID343D2
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF110,279,819110,279,838TGAGGACAGCCCATCTCTTG
TUP110,279,791110,279,817TGTCTTTGATGGTCTTGGCTTCCTGGG
TUR110,279,768110,279,787CCAGTTCACACCGCTATCAC
TDF110,260,926110,260,950TGGTGACTTTGATTGCTTGTGAATG
TDP110,260,899110,260,924TGAAGAGTGACAAGCACCAACACAGC
TDR110,260,827110,260,849CCTCTGATGCTGGTATCCATGAC

PCR Primers

PrimerSequence
SUAGCGCTCTTTGAAGAAATGG
SDCACCAAACACAGGAGCTTGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR71 bp++-
TDFTDR124 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15713A-E10VGB6C57BL/6NTac
15713A-B1VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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