| Gene | Gapt |
| Chromosome | 13 |
| Deletion Start | 110,354,124 |
| Deletion End | 110,353,641 |
| Deletion Size | 484 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,354,040 | 110,354,060 | TGCTCTGTGGGATTGGATGTG |
| TUP | 110,354,016 | 110,354,038 | TTGGCACTGGAATCGCAGGGAAA |
| TUR | 110,353,967 | 110,353,986 | TGCCTGCTGCTTCTCCTTTG |
| TDF | 110,353,788 | 110,353,810 | GACAACTGAGAAGGCCCTGTATG |
| TDP | 110,353,760 | 110,353,783 | CACGCAGCCGTCTAATCTCGAGGA |
| TDR | 110,353,736 | 110,353,756 | TCCGTTTGATTCCCGTAGACG |
PCR Primers
| Primer | Sequence |
| SU | TTGAGGTTTCTGCTGTGCTG |
| SD | ACGACCTGCTAGGAGACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15714A-C2 | VGB6 | C57BL/6NTac | |
| 15714A-D4 | VGB6 | C57BL/6NTac | |
| 15714A-G2 | VGB6 | C57BL/6NTac | |
| 15714A-H1 | VGB6 | C57BL/6NTac | |
| 15714A-H3 | VGB6 | C57BL/6NTac | |
