| Gene | Gzma |
| Chromosome | 13 |
| Deletion Start | 113,100,948 |
| Deletion End | 113,093,876 |
| Deletion Size | 7,073 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,100,277 | 113,100,297 | GACCATGCACTGGTGGGAATG |
| TUP | 113,100,249 | 113,100,274 | ATCATTGTTCTTGCCCTGACCCGGGA |
| TUR | 113,100,189 | 113,100,212 | GCCAATCTTCCTACCAGGTTCTTC |
| TDF | 113,094,695 | 113,094,712 | TCCCATGTCCTGCCTTCC |
| TDP | 113,094,672 | 113,094,693 | TTAGGGCACCCAGGAGCCTGAC |
| TDR | 113,094,646 | 113,094,666 | TGGTGCATGTGATTGCCTGAG |
PCR Primers
| Primer | Sequence |
| SU | CAAAGAGCTCCTACTAAGGGACC |
| SD | CTAGGTGTTCCTGGGCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15716A-A10 | VGB6 | C57BL/6NTac | |
| 15716A-H8 | VGB6 | C57BL/6NTac | |
| 15716A-E8 | VGB6 | C57BL/6NTac | |
| 15716A-E5 | VGB6 | C57BL/6NTac | |
| 15716A-C5 | VGB6 | C57BL/6NTac | |
| 15716A-E2 | VGB6 | C57BL/6NTac | |
