| Gene | Plau |
| Chromosome | 14 |
| Deletion Start | 20,842,528 |
| Deletion End | 20,837,082 |
| Deletion Size | 5,447 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,837,251 | 20,837,274 | GCTTTCCCAGGAAATAGGACAATG |
| TUP | 20,837,275 | 20,837,297 | TATCAGCGGAGGGCTTGTGCACC |
| TUR | 20,837,312 | 20,837,333 | TTCTTCCTCCCTTGCCAGATAG |
| TDF | 20,842,390 | 20,842,412 | CTCACACTTCCTGGACTGGATTC |
| TDP | 20,842,416 | 20,842,443 | CCCACATTGGAGAAGAGAAAGGTCTGGC |
| TDR | 20,842,460 | 20,842,479 | TGTTTCTTCCCTCAGCTACC |
PCR Primers
| Primer | Sequence |
| SU | TGCTGTTGGTCTGAAGCAAG |
| SD | CTGTTCCCTCAAGCCGTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15723A-C2 | VGB6 | C57BL/6NTac | |
| 15723A-C12 | VGB6 | C57BL/6NTac | |
| 15723A-D6 | VGB6 | C57BL/6NTac | |
| 15723A-H1 | VGB6 | C57BL/6NTac | |
| 15723A-A12 | VGB6 | C57BL/6NTac | |
