| Gene | Nfatc4 |
| Chromosome | 14 |
| Deletion Start | 55,833,714 |
| Deletion End | 55,825,040 |
| Deletion Size | 8,675 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,825,885 | 55,825,903 | AGAGCCTGGGCAAGCACAC |
| TUP | 55,825,908 | 55,825,935 | AGGCCTGGCTCTAGAATTCCCCAAACCA |
| TUR | 55,825,962 | 55,825,986 | ATGCCAGGACCTCATACTCTAACAC |
| TDF | 55,833,272 | 55,833,294 | CTCTTTCTCCCAACTGACCACAC |
| TDP | 55,833,297 | 55,833,322 | ACTTCTGACCTGCTGCCACAGATGTA |
| TDR | 55,833,337 | 55,833,356 | CCCTGCATCTTGGCCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GGCCAATAGGTACCATTAG |
| SD | CCTCTCAACGTCTCAGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15734A-B9 | VGB6 | C57BL/6NTac | |
| 15734A-C3 | VGB6 | C57BL/6NTac | |
| 15734A-E2 | VGB6 | C57BL/6NTac | |
| 15734A-E5 | VGB6 | C57BL/6NTac | |
| 15734A-G5 | VGB6 | C57BL/6NTac | |
