| Gene | Atp12a |
| Chromosome | 14 |
| Deletion Start | 56,376,268 |
| Deletion End | 56,365,284 |
| Deletion Size | 10,985 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,365,474 | 56,365,493 | TGTGCAAGAAGGGTCCTCAG |
| TUP | 56,365,494 | 56,365,517 | AGTTGCCAAGCACAGCAGCTGTTC |
| TUR | 56,365,539 | 56,365,559 | GCATCCAACAGAACCCAAGTC |
| TDF | 56,375,945 | 56,375,963 | GCGCCATTGCTTCATGTTC |
| TDP | 56,375,966 | 56,375,988 | ACATGCAATCCTGCAATCGCCGA |
| TDR | 56,375,993 | 56,376,012 | GACAAAGTCACCACCGAGTG |
PCR Primers
| Primer | Sequence |
| SU | TCCAGCCAGGTATCAAGAGG |
| SD | CATCTGCAGTAGGAAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15737A-B4 | VGB6 | C57BL/6NTac | |
| 15737A-G9 | VGB6 | C57BL/6NTac | |
| 15737A-B12 | VGB6 | C57BL/6NTac | |
| 15737A-C2 | VGB6 | C57BL/6NTac | |
| 15737A-F10 | VGB6 | C57BL/6NTac | |
| 15737A-C5 | VGB6 | C57BL/6NTac | |
