| Gene | Blk |
| Chromosome | 14 |
| Deletion Start | 63,387,823 |
| Deletion End | 63,373,290 |
| Deletion Size | 14,534 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,387,687 | 63,387,704 | TCCGGGACAGGGTCTAAG |
| TUP | 63,387,659 | 63,387,678 | TGCTCCCTGAAGCGGCTGCT |
| TUR | 63,387,615 | 63,387,634 | GGCAGGGAATGGAACGAAAC |
| TDF | 63,373,458 | 63,373,476 | GGAGCGGCCTACCTTTGAG |
| TDP | 63,373,432 | 63,373,457 | TTCCTGCAGTCGGTGTTGGAGGACTT |
| TDR | 63,373,367 | 63,373,387 | GGAGCCCTGAAGAGACCATTG |
PCR Primers
| Primer | Sequence |
| SU | TCGCCATTGACAGAGGAG |
| SD | CCTGGAGGATTGACCCATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15739A-C5 | VGB6 | C57BL/6NTac | |
| 15739A-H8 | VGB6 | C57BL/6NTac | |
| 15739A-F4 | VGB6 | C57BL/6NTac | |
| 15739A-H2 | VGB6 | C57BL/6NTac | |
| 15739A-G8 | VGB6 | C57BL/6NTac | |
| 15739A-H1 | VGB6 | C57BL/6NTac | |
