| Gene | Enox1 |
| Chromosome | 14 |
| Deletion Start | 77,508,270 |
| Deletion End | 77,506,881 |
| Deletion Size | 1,390 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,507,013 | 77,507,033 | TCAGCAGTGAAAGTCCATGTG |
| TUP | 77,507,035 | 77,507,052 | AATCAGGGGCCAGGGTCT |
| TUR | 77,507,061 | 77,507,085 | TTTCCTTGAGTTTCATAGTGTCCTC |
| TDF | 77,508,093 | 77,508,111 | GAGCCTTGCCTGTCAACAC |
| TDP | 77,508,116 | 77,508,140 | CTCAGTTTGTTTCTCCCGCAGCAGC |
| TDR | 77,508,156 | 77,508,178 | GCTGGGTGGTATCTAGGGCTATG |
PCR Primers
| Primer | Sequence |
| SU | AGAAATCACGTTCACCCTGG |
| SD | GCACTGTACAGGACTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15747A-A2 | VGB6 | C57BL/6NTac | |
| 15747A-B7 | VGB6 | C57BL/6NTac | |
| 15747A-C5 | VGB6 | C57BL/6NTac | |
| 15747A-D8 | VGB6 | C57BL/6NTac | |
