| Gene | Tdrd3 |
| Chromosome | 14 |
| Deletion Start | 87,487,283 |
| Deletion End | 87,472,166 |
| Deletion Size | 15,118 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,472,870 | 87,472,891 | GCCTGGAATTAGCAGAGATGTG |
| TUP | 87,472,902 | 87,472,931 | TGTGTTGCTGATAGTTACTGCTGATCTCGG |
| TUR | 87,472,952 | 87,472,971 | CGGGAGTCAAAGGGCAGTTG |
| TDF | 87,486,334 | 87,486,357 | GCCCTCCTGCTAGAGGTATAGTTC |
| TDP | 87,486,361 | 87,486,385 | TGCCAGCAAGATATGGGAAGGACCA |
| TDR | 87,486,455 | 87,486,474 | GCCTGCATGACCTGTATGTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGCTTTCTTAGTGCTTTCG |
| SD | CAGACAGACCAATCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 141 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15748C-E11 | VGB6 | C57BL/6NTac | |
