| Gene | C9 |
| Chromosome | 15 |
| Deletion Start | 6,459,668 |
| Deletion End | 6,445,365 |
| Deletion Size | 14,304 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,445,453 | 6,445,474 | TCAATGCACAGATGCCAATACC |
| TUP | 6,445,475 | 6,445,503 | CGTTTCCAGGTGAGTCCATAGCTTGTCTG |
| TUR | 6,445,527 | 6,445,546 | AAGCCCTGGACAGCAGATTC |
| TDF | 6,459,556 | 6,459,575 | TGGTGACAACGACTGTGGAG |
| TDP | 6,459,581 | 6,459,610 | TCTGATGAGAATGACTGTGACGATGACCCA |
| TDR | 6,459,630 | 6,459,652 | CAGCTCTGATTCTTCCGCTACTC |
PCR Primers
| Primer | Sequence |
| SU | TCTTTAGCTTCCCTTCCAGAG |
| SD | CTTCAATGGACTTGACACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15754B-C9 | VGB6 | C57BL/6NTac | |
| 15754B-G1 | VGB6 | C57BL/6NTac | |
| 15754B-H3 | VGB6 | C57BL/6NTac | |
| 15754C-G11 | VGB6 | C57BL/6NTac | |
| 15754B-E2 | VGB6 | C57BL/6NTac | |
| 15754C-C2 | VGB6 | C57BL/6NTac | |
