| Gene | Ncald |
| Chromosome | 15 |
| Deletion Start | 37,397,431 |
| Deletion End | 37,368,812 |
| Deletion Size | 28,620 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,397,129 | 37,397,150 | CAAGCTGGAGCAGAAGCTGAAG |
| TUP | 37,397,104 | 37,397,128 | TGGGCCTTCAGCATGTATGACCTGG |
| TUR | 37,397,078 | 37,397,099 | TGCCTTGCTGATGTAGCCATTG |
| TDF | 37,369,218 | 37,369,238 | ACCAGTGTGGAGTGGCTCAAG |
| TDP | 37,369,193 | 37,369,217 | AGCCTGGCACACTCAGATGAGCTGG |
| TDR | 37,369,141 | 37,369,163 | TCTCTGCCCTAGTAGCCTTTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTACCTTCCTTCCATTTGTC |
| SD | GCATGTGTGACAACAGACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15762A-A6 | VGB6 | C57BL/6NTac | |
| 15762A-E1 | VGB6 | C57BL/6NTac | |
| 15762A-E7 | VGB6 | C57BL/6NTac | |
