| Gene | Hsf1 |
| Chromosome | 15 |
| Deletion Start | 76,500,666 |
| Deletion End | 76,477,604 |
| Deletion Size | 23,063 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,478,365 | 76,478,385 | GGAACCTTTGGAGGGATGGAG |
| TUP | 76,478,387 | 76,478,409 | CGGCTAGGTGCCACCACCTTGAC |
| TUR | 76,478,410 | 76,478,430 | GCCAGATTTGGCTTGCAGAAG |
| TDF | 76,499,942 | 76,499,961 | GGCCAGGAACGAGCTAAGTG |
| TDP | 76,499,963 | 76,499,985 | TCACCTGGATGCCATGGACTCCA |
| TDR | 76,500,002 | 76,500,019 | TGGCTTGTCAGCATGGTC |
PCR Primers
| Primer | Sequence |
| SU | ACCAGGCTCTGATTGGTGAG |
| SD | AGCTCTGCCTACATTCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15769A-H2 | VGB6 | C57BL/6NTac | |
| 15769A-B10 | VGB6 | C57BL/6NTac | |
| 15769A-G9 | VGB6 | C57BL/6NTac | |
| 15769A-E3 | VGB6 | C57BL/6NTac | |
| 15769A-C10 | VGB6 | C57BL/6NTac | |
| 15769A-G8 | VGB6 | C57BL/6NTac | |
