| Gene | Foxred2 |
| Chromosome | 15 |
| Deletion Start | 77,956,085 |
| Deletion End | 77,943,069 |
| Deletion Size | 13,017 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,955,627 | 77,955,648 | AGCCTGGAACGGCCATTACTTC |
| TUP | 77,955,604 | 77,955,625 | TTCTGACGGACCAGAAGGGCCA |
| TUR | 77,955,569 | 77,955,588 | TGTGTCCTGAGGCTCCTTAC |
| TDF | 77,943,433 | 77,943,452 | AGCTCCTCTCCGTGCTTGTC |
| TDP | 77,943,410 | 77,943,431 | CCCAAGCCTGGCCTGACACCTC |
| TDR | 77,943,360 | 77,943,379 | TCTGGCGTGTGAGTGTGAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGGTAGACCAACCAGAACC |
| SD | CTGAAGACTCTAAACCTTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15770A-B4 | VGB6 | C57BL/6NTac | |
| 15770A-E12 | VGB6 | C57BL/6NTac | |
| 15770A-E1 | VGB6 | C57BL/6NTac | |
| 15770A-G9 | VGB6 | C57BL/6NTac | |
