| Gene | Cdc42ep1 |
| Chromosome | 15 |
| Deletion Start | 78,850,009 |
| Deletion End | 78,847,360 |
| Deletion Size | 2,650 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,847,696 | 78,847,716 | CCCATCATCAAGAACGCCATC |
| TUP | 78,847,719 | 78,847,740 | CCTGCCTCAGCTCAACCAGGCC |
| TUR | 78,847,751 | 78,847,768 | GCTTGCCCATCACCAGAC |
| TDF | 78,849,843 | 78,849,862 | CCAGTGTCAGGGCTCCTGTG |
| TDP | 78,849,865 | 78,849,887 | CACCTCGGTGCAGGTCAATGCCT |
| TDR | 78,849,908 | 78,849,929 | ACACCTTGACCTCGTCATCTTC |
PCR Primers
| Primer | Sequence |
| SU | AGCCTTGTTTGTTCTCTCGG |
| SD | CAATCAGGTATGGGAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15771A-C6 | VGB6 | C57BL/6NTac | |
