| Gene | Grap2 |
| Chromosome | 15 |
| Deletion Start | 80,648,603 |
| Deletion End | 80,623,740 |
| Deletion Size | 24,864 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,623,950 | 80,623,970 | ACCAGATGTGGGCACATGCTC |
| TUP | 80,623,975 | 80,623,999 | TCCTAGCACTCGAGAGGTAGAGGCA |
| TUR | 80,624,007 | 80,624,027 | AGGCTGGCCTTGGAACTCTTG |
| TDF | 80,648,486 | 80,648,507 | CGAAGCGGAGAAGTGGTTGAAG |
| TDP | 80,648,511 | 80,648,534 | TGGACAGCTCCAACCCATCTTGGT |
| TDR | 80,648,554 | 80,648,574 | GCAGGGAAGAGACCCAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | TCCAGTTGATAGCAAGCCTTC |
| SD | CAGGAAGGAAACGGTGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15773A-A7 | VGB6 | C57BL/6NTac | |
| 15773A-D9 | VGB6 | C57BL/6NTac | |
| 15773A-G11 | VGB6 | C57BL/6NTac | |
| 15773A-H8 | VGB6 | C57BL/6NTac | |
| 15773A-H9 | VGB6 | C57BL/6NTac | |
