| Gene | Gxylt1 |
| Chromosome | 15 |
| Deletion Start | 93,275,078 |
| Deletion End | 93,259,069 |
| Deletion Size | 16,010 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,274,271 | 93,274,290 | TAATTCGCACTGCCTCCTTG |
| TUP | 93,274,243 | 93,274,269 | TGGATTCCTGTGTTTGGGCAGTTCTGA |
| TUR | 93,274,208 | 93,274,230 | ACAATTCCTGTCTTCCACTCTCC |
| TDF | 93,259,547 | 93,259,566 | GGTTCCTGGACAGGCTGATG |
| TDP | 93,259,519 | 93,259,545 | CCGAGCAAGCGTAGTTGTTTCCCTGTC |
| TDR | 93,259,463 | 93,259,486 | CCCAGAACGCTAAGAATGAGAATC |
PCR Primers
| Primer | Sequence |
| SU | AGCATCCATCTCAAGGCTCG |
| SD | CACCAGGACCTGCTCTATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15783A-D8 | VGB6 | C57BL/6NTac | |
| 15783A-H10 | VGB6 | C57BL/6NTac | |
