| Gene | 4732456N10Rik |
| Chromosome | 15 |
| Deletion Start | 101,562,822 |
| Deletion End | 101,553,146 |
| Deletion Size | 9,677 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,562,174 | 101,562,195 | TCCATCCATCCCGAGTTCTTAG |
| TUP | 101,562,145 | 101,562,172 | CCCACAACAAAGACACTGCTACTCTGGT |
| TUR | 101,562,097 | 101,562,118 | TTATTGCCTTCCCTCCAATCTG |
| TDF | 101,553,734 | 101,553,751 | ACAGCCCAGGAGCCTAAG |
| TDP | 101,553,709 | 101,553,732 | CCTGGACTCAGGCAGAGGTGACCT |
| TDR | 101,553,677 | 101,553,700 | TGGAGGTGCAAAGAGATCAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTGTGGATGGAACAGTCC |
| SD | TTTAGCCAGGACAAGGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15786A-D2 | VGB6 | C57BL/6NTac | |
| 15786A-F4 | VGB6 | C57BL/6NTac | |
| 15786A-F6 | VGB6 | C57BL/6NTac | |
