| Gene | Hoxc6 |
| Chromosome | 15 |
| Deletion Start | 103,011,115 |
| Deletion End | 103,009,609 |
| Deletion Size | 1,507 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,009,703 | 103,009,726 | CAGTGAGGCATTTCTCGACCTATG |
| TUP | 103,009,728 | 103,009,750 | AGCAGCCGTAGCTCAGAACCGGA |
| TUR | 103,009,782 | 103,009,803 | GCTGGAACTGAACACGACATTC |
| TDF | 103,010,846 | 103,010,865 | CATCGAGATCGCCAATGCTC |
| TDP | 103,010,866 | 103,010,888 | TGTGCCTGACCGAGCGACAGATC |
| TDR | 103,010,901 | 103,010,918 | CTTCATGCGGCGGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | GAACAAGGCCCTGCTCATAG |
| SD | AGCCTAGGGCTCACTTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15792A-A4 | VGB6 | C57BL/6NTac | |
| 15792A-A6 | VGB6 | C57BL/6NTac | |
| 15792A-D1 | VGB6 | C57BL/6NTac | |
| 15792A-D6 | VGB6 | C57BL/6NTac | |
| 15792A-E11 | VGB6 | C57BL/6NTac | |
| 15792A-G9 | VGB6 | C57BL/6NTac | |
