| Gene | Pde1b |
| Chromosome | 15 |
| Deletion Start | 103,528,387 |
| Deletion End | 103,503,477 |
| Deletion Size | 24,911 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,503,825 | 103,503,843 | TTGTGTTTGCGGCCCTTTG |
| TUP | 103,503,846 | 103,503,865 | TGACAGGCTGACACGCAGCA |
| TUR | 103,503,869 | 103,503,887 | ACAGTGCGCTGCCTCTTTG |
| TDF | 103,527,872 | 103,527,894 | CCACAGGAAAGGCTGTCTGAATG |
| TDP | 103,527,897 | 103,527,921 | TTGGGCTGCTCTAGAACGAGAGCTG |
| TDR | 103,527,924 | 103,527,946 | ACTATGGCTGTCCCTATCATCTC |
PCR Primers
| Primer | Sequence |
| SU | GAGGAGTGGCTACACTGCG |
| SD | TCTCCAAATTCCACCCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15793A-A8 | VGB6 | C57BL/6NTac | |
| 15793A-B8 | VGB6 | C57BL/6NTac | |
| 15793A-H10 | VGB6 | C57BL/6NTac | |
| 15793A-H4 | VGB6 | C57BL/6NTac | |
