| Gene | Tfap4 |
| Chromosome | 16 |
| Deletion Start | 4,559,513 |
| Deletion End | 4,545,498 |
| Deletion Size | 14,016 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,559,490 | 4,559,511 | GTATTTCATGGTGCCCACTCAG |
| TUP | 4,559,462 | 4,559,489 | AAGGTGCCCTCTTTGCAACATTTCAGGA |
| TUR | 4,559,430 | 4,559,450 | ACAGAGCCCTCCTATCACTTC |
| TDF | 4,545,700 | 4,545,721 | GGACAAACAGGAGCTGGAAGAG |
| TDP | 4,545,671 | 4,545,698 | AACAGAGGCGAGCAGTTATCGTGAAGTC |
| TDR | 4,545,628 | 4,545,646 | CGAGGCGGTATCAGAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TTCCTTACTCCGCGGAAG |
| SD | ATAACCCGACAGAGGGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15795A-B8 | VGB6 | C57BL/6NTac | |
| 15795A-F2 | VGB6 | C57BL/6NTac | |
| 15795A-G5 | VGB6 | C57BL/6NTac | |
| 15795A-H2 | VGB6 | C57BL/6NTac | |
| 15795A-H4 | VGB6 | C57BL/6NTac | |
| 15795A-G9 | VGB6 | C57BL/6NTac | |
