| Gene | Cldn26 |
| Chromosome | 16 |
| Deletion Start | 8,424,649 |
| Deletion End | 8,409,533 |
| Deletion Size | 15,117 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,424,409 | 8,424,426 | CTTCCAGGGTCCGTGTTC |
| TUP | 8,424,385 | 8,424,407 | CACCTGGGTGCAGTACCTGGGAC |
| TUR | 8,424,349 | 8,424,373 | TTGGAGGTTGAGATAGGAGTATGAG |
| TDF | 8,409,785 | 8,409,806 | GCAGACCAAGAGGTCCTGAATG |
| TDP | 8,409,760 | 8,409,782 | TGTCCCTGTCTTTCCCTGCAGCC |
| TDR | 8,409,704 | 8,409,721 | CGGAAGGCAACTGCTGAG |
PCR Primers
| Primer | Sequence |
| SU | GGACTGAGGTCGACAGAG |
| SD | AGCTGATCCAAAGGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15800A-A9 | VGB6 | C57BL/6NTac | |
| 15800A-D6 | VGB6 | C57BL/6NTac | |
| 15800A-D9 | VGB6 | C57BL/6NTac | |
| 15800A-F4 | VGB6 | C57BL/6NTac | |
| 15800A-E1 | VGB6 | C57BL/6NTac | |
