15803   Efcab1     Status: ES cell colonies screened / QC positives

GeneEfcab1
Chromosome16
Deletion Start14,924,211
Deletion End14,906,758
Deletion Size17,454
Genome Build38
MGI IDMGI:1914043
NCBI Gene ID66793
BAC ID431C9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF14,906,80314,906,826TGCAAGCACTGTAAGTAAGAACTG
TUP14,906,82914,906,857TGCAAGAGTTCCTATGACTTCTGGGCCAG
TUR14,906,87514,906,895TGGTGGAAGCAGGAAGTAGAG
TDF14,923,71714,923,741CTGGTCTTCAGTATTCATTGGGTTC
TDP14,923,75114,923,779TAGACTCCAATCCCAGTGAAGGAATCCAC
TDR14,923,78714,923,805TTGCTTGCCCTCACTTTCC

PCR Primers

PrimerSequence
SUAAATGCAGGCACAGTGTCTG
SDCCATGTATCCTTCATCTTGCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR93 bp++-
TDFTDR89 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15803A-A9VGB6C57BL/6NTac
15803A-D9VGB6C57BL/6NTac
15803A-H1VGB6C57BL/6NTac
15803A-F12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image