| Gene | P2rx6 |
| Chromosome | 16 |
| Deletion Start | 17,570,980 |
| Deletion End | 17,562,047 |
| Deletion Size | 8,934 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,562,364 | 17,562,383 | GCTTTGTCTCCAGCGTCTTC |
| TUP | 17,562,384 | 17,562,410 | TCGGTCTCTGTTAGCGTCTGCAGTTCT |
| TUR | 17,562,436 | 17,562,458 | GCTCAGAGAATTACTGCCTCCTG |
| TDF | 17,570,468 | 17,570,489 | GGGAAGGATTTGTGGAGGTTTG |
| TDP | 17,570,492 | 17,570,517 | AGGACAACCACAGTCCGAGAGCTTCA |
| TDR | 17,570,548 | 17,570,567 | GTGATGGCCGTAGGGATGAG |
PCR Primers
| Primer | Sequence |
| SU | CACTGCCTAAGGGTCTCAGC |
| SD | ACTTATTCAGCCACATCGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15806A-B2 | VGB6 | C57BL/6NTac | |
| 15806A-B8 | VGB6 | C57BL/6NTac | |
| 15806A-D3 | VGB6 | C57BL/6NTac | |
| 15806A-D6 | VGB6 | C57BL/6NTac | |
| 15806A-D10 | VGB6 | C57BL/6NTac | |
| 15806A-D8 | VGB6 | C57BL/6NTac | |
