| Gene | Tprg |
| Chromosome | 16 |
| Deletion Start | 25,328,349 |
| Deletion End | 25,317,264 |
| Deletion Size | 11,086 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,317,338 | 25,317,359 | TGACCACTTGTCCACAAAGGAG |
| TUP | 25,317,366 | 25,317,389 | TCAGGCAAGGACCCAGGATCAAGG |
| TUR | 25,317,434 | 25,317,453 | AGGGCTGGCGATAAGGATTG |
| TDF | 25,328,267 | 25,328,286 | GGGCCATTGAGACTGCTGTG |
| TDP | 25,328,294 | 25,328,321 | TGAAAGGCCACATAGCACAGACTTCTGG |
| TDR | 25,328,329 | 25,328,349 | CAGGAGCCAGAAGCCTTGAAC |
PCR Primers
| Primer | Sequence |
| SU | ATGCTACATTTCCTATGTTCAG |
| SD | TGTAGTTACAGAGAGCATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15812A-A1 | VGB6 | C57BL/6NTac | |
| 15812A-E10 | VGB6 | C57BL/6NTac | |
| 15812A-H7 | VGB6 | C57BL/6NTac | |
| 15812A-B5 | VGB6 | C57BL/6NTac | |
