| Gene | Snx4 |
| Chromosome | 16 |
| Deletion Start | 33,280,701 |
| Deletion End | 33,251,464 |
| Deletion Size | 29,238 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,252,096 | 33,252,115 | GGAGTGTCGCGTTGTCAATC |
| TUP | 33,252,118 | 33,252,142 | TGAGTGCATCCATCCAAAGGGTCGT |
| TUR | 33,252,194 | 33,252,217 | GAGGGTCAGGGAGTTCAAATACAG |
| TDF | 33,280,033 | 33,280,054 | TGGTGTCAGGCTAGAAAGATGG |
| TDP | 33,280,058 | 33,280,086 | CGTAGGTTAGAGCACTTGCTGATCTTCCA |
| TDR | 33,280,121 | 33,280,141 | GGAGGGAGCTACGAGTGATTG |
PCR Primers
| Primer | Sequence |
| SU | TGATGCTGCTCTGGTCTTTG |
| SD | CATTCCACTCTGAAATGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15814A-H9 | VGB6 | C57BL/6NTac | |
| 15814A-C9 | VGB6 | C57BL/6NTac | |
| 15814A-C11 | VGB6 | C57BL/6NTac | |
| 15814A-D3 | VGB6 | C57BL/6NTac | |
| 15814A-H2 | VGB6 | C57BL/6NTac | |
| 15814A-A8 | VGB6 | C57BL/6NTac | |
