| Gene | Muc13 |
| Chromosome | 16 |
| Deletion Start | 33,815,870 |
| Deletion End | 33,794,530 |
| Deletion Size | 21,341 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,795,279 | 33,795,300 | CCTTGGAGTGCTGGGTTATAGG |
| TUP | 33,795,303 | 33,795,331 | TGAGCCAACATACATGGCAAGATAGGAGC |
| TUR | 33,795,374 | 33,795,393 | TCTGGGAGTTGTCCGATGTC |
| TDF | 33,815,705 | 33,815,728 | CAGAGGCTGATTGAGGATGACTTC |
| TDP | 33,815,730 | 33,815,756 | ATAACCTACGACTGAGGCAGACCGGCT |
| TDR | 33,815,764 | 33,815,783 | TGCTGTTGTCCGCTCCTAAG |
PCR Primers
| Primer | Sequence |
| SU | GGCGATAGGTCTACCATTCC |
| SD | GAGGCACTCAAGGATGCTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 115 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15815A-C3 | VGB6 | C57BL/6NTac | |
| 15815A-D2 | VGB6 | C57BL/6NTac | |
| 15815A-D4 | VGB6 | C57BL/6NTac | |
| 15815A-E5 | VGB6 | C57BL/6NTac | |
| 15815A-F4 | VGB6 | C57BL/6NTac | |
| 15815A-G1 | VGB6 | C57BL/6NTac | |
